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Levicyn HOCl 2017-12-21T19:14:04+00:00

Benefits of HOCl

Stabilized Hypochlorous acid (HOCl) helps reduce itch, inflammation and pain. It is also a powerful antimicrobial that kills skin pathogens associated with atopic dermatitis.1-4

What are the Key Clinical Benefits of HOCl?

  • Potent antimicrobial1
  • Reduction of pain and itch2,3
  • Reduction of inflammation4
  • Increased oxygenation of wound site5
  • Breaks down biofilm formed by S. aureus6
  • Helps with wound healing6

Potent Antimicrobial1

Time Kill Study: In Vitro in solution HOCl Eradication of Pathogens

  • Greater than 99% reduction in killing 23 different microorganisms, including S. aureus and S. aureus-MRSA
  • Time to kill 30 seconds
 
Name of Organism Log Reduction Log Reduction Percent Reduction
Acinetobacter baumannii 7.0488 30 seconds 99.9999%
Bacteroides fragilis 3.3565 30 seconds 99.9560%
Candida albicans 6.4699 30 seconds 99.9999%
Corynebacterium diphtherieae 5.2778 30 seconds 99.9995%
Clostridium difficile (spores) 4.6085 30 seconds 99.9975%
Enterobacter aerogenes 6.7200 30 seconds 99.9999%
Enterococcus faecalis-VRE MDR 5.6085 30 seconds 99.9998%
Escherichia coli 6.5272 30 seconds 99.9999%
Haemophilus influenzae 5.4107 30 seconds 99.9996%
Klebsiella oxytoca 6.7810 30 seconds 99.9999%
Klebsiella pneumoniae ozaenae
6.8086 30 seconds 99.9999%
Malassezia furfur 5.3953 30 seconds 99.9996%
Micrococcus yunnanensis 5.6137 30 seconds 99.9998%
Propionibacterium acnes 5.2967 30 seconds 99.9995%
Proteus mirabilis 6.7477 30 seconds 99.9999%
Pseudomonas aeruginosa 6.5867 30 seconds 99.9999%
Serratia marcescens 6.5811 30 seconds 99.9999%
Staphylococcus aureus-MRSA 6.7032 30 seconds 99.9999%
Staphylococcus aureus 6.5008 30 seconds 99.9999%
Staphylococcus pyogenes
6.4214 30 seconds 99.9999%
Trichophyton mentagrophytes
4.6752 30 seconds 99.9979%
Trichophyton rubrum
2.5234 30 seconds 99.7004%
Vibrio vulnificus
5.1888 30 seconds 99.9994%

Reduction in Pain and Itch

Study 1: Open-Label Study2

Study Design

  • Open-label, single-arm study over 7 days

Results

  • HOCl rapidly reduces pruritus in mild-to- moderate atopic dermatitis (AD)
    • 50% of patients experienced a reduction by Day 1
    • 85% of patients experienced a reduction by Day 3

Study 2: Randomized, Blinded, Controlled Study3

Study Design

  • 72-hour study
  • Treated group (n=19): HOCl BID
  • Untreated group (n=10): No treatment

Results

  • Mean pruritus VAS scores between treated and
    untreated group were significantly different between
    baseline and 72 hours post application

Anti-Inflammatory Activity4

HOCl for Inhibition of Mast Cell Degranulation

  • Prevents allergen and calcium-induced mast cell degranulation for up to 8 hours after a single exposure in vitro
  • Reduces wound inflammation that inhibits healing
  • Stabilizes the mast cell membrane to inhibit the cell machinery for granule secretion, while not altering the signal transduction pathways or calcium mobilization
  • Inhibits degranulation stimulated by antigen or calcium ionophore

 

 

*A23187 + PMA is a calcium ionophore; †b-hexosaminidase is a marker of IgE-antigen-induced mast cell degranulation.

Increases Oxygenation5

  • Provides higher levels of oxygen at a wound site after application
  • Elevated blood and oxygen flow increases wound-healing nutrients

Breaks Down Bacterial Biofilm6

Biofilm formation is recognized as a serious problem in chronic wound infections. Biofilm characteristics include:

  • Complex structure of microorganisms that generate a protective shell, allowing bacteria to collect and proliferate
  • Most microorganisms that form biofilms found growing in microbial infection
  • Adheres to chronic wounds, makes phagocytosis difficult, and increases resistance to antibiotics

HOCl has antibiofilm activity and actively breaks down biofilm and kills microorganisms within the biofilm.

 

 

  • Corresponding microbicidal activity: 1/64 and lower dilutions of HOCl demonstrated zero colony-forming units of S. aureus

Assists Wound Healing6

Wound healing involves a complex sequence of events. Fibroblasts are the primary synthetic cells in the repair process of most structural proteins used during tissue reconstruction.

At lower concentrations, the percentage of fibroblast migration exceeded that of the control at 24 hours.

Effect of HOCl on Skin Fibroblast Cell Migration in a Wound Healing Assay

 

 

Although fibroblasts have a critical role in wound healing, their proliferation highly relates to keratinocyte proliferation and mediators secreted from keratinocytes.

  • Similar results were obtained for keratinocyte migration: At lower HOCl concentrations, the percentage of keratinocyte migration exceeded that of the control at 24 hours

References: 1. Data on File. IntradermPharmaceuticals. 2. Draelos Z, Cash K. Evaluation of a gel formulation of hypochlorous acid and sodium hypochlorite to reduce pruritus in mild to moderate atopic dermatitis. Poster presented at: The Winter Clinical Dermatology Conference. January 14-19, 2012; Maui, HI. 3. Berman B, Nestor M. Investigator blinded randomized study evaluation HOCl in the treatment of atopic dermatitis-associated pruritus. Poster presented at: The Fall Clinical Dermatology Conference. Oct 12-15, 2017; Las Vegas, NV. 4. Medina-Tamayo J, Sánchez-Miranda E, Ballez-Tapia H, et al. Super-oxidized solution inhibits IgE-antigen-induced degranulation and cytokine release in mast cells. Int Immunopharmacol. 2007; 7(8): 1013-2014. 5. Bongiovanni CM. Superoxidized water improves wound care outcomes in diabetic patients. Available at: http://www.microdacyn.pl/pdf/7ART-02-Bongiovanni-DMC-05-2006.pdf. Accessed September 9, 2017. 6. Sakayra S, gunay N, Karakulak M, et al. Hypochlorous acid: An ideal wound care agent with powerful microbicidal, antibiofilm, and wound healing potency. Wounds. 2014;26(12):342-350.